Publications

Manuscripts

  • Giladi M*, Lisnyansky Bar-El M, Vaňková P, Ferofontov A, Melvin E, Alkaderi S, Kavan D, Redko B, Haimov E, Wiener R, Man P, Haitin Y*. Structural basis for long-chain isoprenoid synthesis by cis-prenyltransferases. Science Advances. 2022; 8(20). DOI: 10.1126/sciadv.abn1171.

 

  • Kellner S, Abbasi A, Carmi I, Heinrich R, Garin-Shkolnik T, Hershkovitz T, Giladi M, Haitin Y, Johannesen KM, Steensbjerre Mølle R, Berlin S. Two de novo GluN2B mutations affect multiple NMDAR-functions and instigate severe pediatric encephalopathy. Elife. 2021; 10. DOI: 10.7554/eLife.67555.

  • Lisnyansky Bar-El M, Vaňková P, Yeheskel A, Simhaev L, Engel H, Man P, Haitin Y*, Giladi M*. Structural basis of heterotetrameric assembly and disease mutations in the human cis-prenyltransferase complex. Nature Communications. 2020; 11: 5273. DOI: 10.1038/s41467-020-18970-z.

  • Ferofontov A, Vaňková P, Man P, Giladi M, Haitin Y. Conserved cysteine dioxidation enhances membrane interaction of human Cl- intracellular channel 5. The FASEB Journal. 2020; 34(8):9925–9940. DOI: 10.1096/fj.202000399R.

  • Ben-Bassat A, Giladi M, Haitin Y. Structure of KCNH2 cyclic nucleotide-binding homology domain reveals a functionally vital salt-bridge. Journal of General Physiology. 2020; 152(4). DOI: 10.1085/jgp.201912505.

  • Lisnyansky Bar-El M, Lee SY, Ki AY, Kapelushnik N, Loewenstein A, Chung KY, Schneidman-Duhovny D, Giladi M*, Newman H, Haitin Y*. Structural characterization of full-length human dehydrodolichyl diphosphate synthase using an integrative computational and experimental approach. Biomolecules. 2019; 9(11):660. DOI: 10.3390/biom9110660.

  • Lisnyansky M, Yariv E, Segal O, Marom M, Loewenstein A, Ben-Tal N, Giladi M*, Haitin Y*. Metal coordination is crucial for GGPPS-bisphosphonates interactions: A crystallographic and computational analysis. Molecular Pharmacology. 2019; 96(5):580-588. DOI: 10.1124/mol.119.117499.

  • Lisnyansky M, Kapelushnik N, Ben-Bassat A, Marom M, Loewenstein A, Khananshvili D, Giladi M*, Haitin Y*. Reduced activity of GGPPS mutant is involved in bisphosphonates-induced atypical fractures. Molecular Pharmacology. 2018; 94(6):1391-1400. DOI: 10.1124/mol.118.113670.

  • Ferofontov A, Strulovich R, Marom M, Giladi M, Haitin Y. Inherent flexibility of CLIC6 revealed by crystallographic and solution studies. Scientific Reports. 2018; 8:6882. DOI: 10.1038/s41598-018-25231-z.

  • Meisel E, Tobelaim W, Dvir M, Haitin Y, Peretz A, Attali B. Inactivation gating of Kv7.1 channels does not involve concerted cooperative subunit interactions. Channels. 2018; 16:0-28. DOI: 10.1080/19336950.2018.1441649.

  • Mashahreh B, Hassouna F, Soudah N, Cohen-Kfir E, Strulovich R, Haitin Y, Wiener R. Trans-binding of UFM1 to UBA5 stimulates UBA5 homodimerization and ATP binding. The FASEB Journal. 2018; 32(5):2794–2802. DOI: 10.1096/fj.201701057R.

  • Padala P, Soudah N, Giladi M, Haitin Y, Isupov MN, Wiener R. The Crystal Structure and Conformations of an Unbranched Mixed Tri-Ubiquitin Chain Containing K48 and K63 Linkages. Journal of Molecular Biology. 2017;429(24):3801–3813. DOI: 10.1016/j.jmb.2017.10.027.

  • Tobelaim WS, Dvir M, Lebel G, Cui M, Buki T, Peretz A, Marom M, Haitin Y, Logothetis DE, Hirsch JA, Attali B. Ca2+-Calmodulin and PIP2 interactions at the proximal C-terminus of Kv7 channels. Channels. 2017; 11(6):686-695. DOI: 10.1080/19336950.2017.1388478.

  • Edri I*, Goldenberg M*, Lisnyansky M, Strulovich R, Newman H, Loewenstein A, Khananshvili D, Giladi M, Haitin Y. Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli. Journal of Visualized Experimentations. 2017; 126. DOI: 10.3791/56430.

  • Giladi M#, Lee SY#, Ariely Y, Teldan Y, Granit R, Strulevich R, Haitin Y, Chung KY*, Khananshvili. D*. Structure-encoded dynamics of regulatory diversity in sodium-calcium exchanger (NCX) isoforms. Scientific Reports. 2017;  7:993. DOI: 10.1038/s41598-017-01102-x.

  • James ZM#, Borst AJ#, Haitin Y, Frenz B, DiMaio F, Zagotta WN, Veesler D. CryoEM Structure of a Prokaryotic Cyclic Nucleotide-Gated Ion Channel. PNAS. 2017; 114(17):4430-4435. DOI: 10.1073/pnas.1700248114.

  • Giladi M*, Edri I, Goldenberg M, Newman H, Strulovich R, Khananshvili D, Haitin Y*, Loewenstein A. Purification and characterization of human dehydrodolychil diphosphate synthase (DHDDS) overexpressed in E. Coli. Protein Expression and Purification. 2017; 132: 138-142. DOI: 10.1016/j.pep.2017.02.001

  • Tobelaim WS, Dvir M, Lebel G, Cui M, Buki T, Peretz A, Marom M, Haitin Y, Logothetis DE, Hirsch JA, Attali B. Competition of calcified calmodulin N lobe and PIP2 to an LQT mutation site in Kv7.1 channel. PNAS. 2017; 114(5):E869-E878. DOI: 10.1073/pnas.1612622114

  • Dvir M, Peretz A, Haitin Y, Attali B. Recent molecular insights from mutated IKS channels in cardiac arrhythmia. Current opinion in pharmacology. 2014;15C:74–82. DOI: 10.1016/j.coph.2013.12.004

  • Dvir M, Strulovich R, Sachyani D, Ben-Tal Cohen I, Haitin Y, Dessauer C, Pongs O, Kass R, Hirsch JA, Attali B. Long QT mutations disrupt IKS regulation by PKA and PIP2 at the same KCNQ1 helix C-KCNE1 interface. Journal of cell science. 2014; 127(18):3943-3955. DOI: 10.1242/jcs.147033.

  • Haitin Y. Structural biology: A “funny” cyclic dinucleotide receptor. Nature chemical biology. 2014;10(6):413–414. DOI: 10.1038/nchembio.1530.

  • Haitin Y, Carlson AE, Zagotta WN. The structural mechanism of KCNH-channel regulation by the eag domain. Nature. 2013;501(7467):444–448. DOI: 10.1038/nature12487.

  • Meisel E, Dvir M, Haitin Y, Giladi M, Peretz A, Attali B. KCNQ1 channels do not undergo concerted but sequential gating transitions in both the absence and the presence of KCNE1 protein. Journal of Biological Chemistry. 2012;287(41):34212–34224. DOI: 10.1074/jbc.M112.364901.

  • Shuart NG, Haitin Y, Camp SS, Black KD, Zagotta WN. Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels. Nature communications. 2011;2:457. DOI: 10.1038/ncomms1466.

  • Peretz A, Pell L, Gofman Y, Haitin Y, Shamgar L, Patrich E, Kornilov P, Gourgy-Hacohen O, Ben-Tal N, Attali B. Targeting the voltage sensor of Kv7.2 voltage-gated K+ channels with a new gating-modifier. PNAS. 2010;107(35):15637–15642. DOI: 10.1073/pnas.0911294107.

  • Haitin Y, Wiener R, Shaham D, Peretz A, Cohen EB-T, Shamgar L, Pongs O, Hirsch JA, Attali B. Intracellular domains interactions and gated motions of IKS potassium channel subunits. The EMBO journal. 2009;28(14):1994–2005. DOI: 10.1038/emboj.2009.157.

  • Haitin Y, Attali B. The C-terminus of Kv7 channels: a multifunctional module. The Journal of physiology. 2008;586(7):1803–1810. DOI: 10.1113/jphysiol.2007.149187.

  • Haitin Y, Yisharel I, Malka E, Shamgar L, Schottenlndreier H, Peretz A, Paas Y, Attali B. S1 constrains S4 in the voltage sensor domain of Kv7.1 K+ channels. PLoS ONE. 2008;3(4):e1935. DOI: 10.1371/journal.pone.0001935.

  • Shamgar L, Haitin Y, Yisharel I, Malka E, Schotteindreier H, Peretz A, Paas Y, Attali B. KCNE1 constrains the voltage sensor of Kv7.1 K+ channels. PLoS ONE. 2008;3(4):e1943. DOI: 10.1371/journal.pone.0001943.

  • Wiener R, Haitin Y, Shamgar L, Fernández-Alonso MC, Martos A, Chomsky-Hecht O, Rivas G, Attali B, Hirsch JA. The KCNQ1 (Kv7.1) COOH terminus, a multitiered scaffold for subunit assembly and protein interaction. The Journal of biological chemistry. 2008;283(9):5815–5830.DOI: 10.1074/jbc.M707541200.

  • Shamgar L, Ma L, Schmitt N, Haitin Y, Peretz A, Wiener R, Hirsch J, Pongs O, Attali B. Calmodulin is essential for cardiac IKS channel gating and assembly: impaired function in long-QT mutations. Circulation research. 2006;98(8):1055–1063. DOI: 10.1161/01.RES.0000218979.40770.69.

  • Giladi M*, Bar-El Lisnyansky M, Vankova P, Ferofontov A, Melvin E, Kavan D, Redko B, Haimov E, Wiener R, Man P, Haitin Y*. Structural basis for long-chain isoprenoids synthesis by cis-prenyltransferases. bioRxiv. 2021:465316. DOI: 10.1101/2021.10.21.465316.

  • Melvin E, Shlush E, Giladi M*, Haitin Y*. TTYH family members form tetrameric complexes within the cell membrane. bioRxiv. 2021:459021. DOI: 10.1101/2021.09.05.459021.

  • Lisnyansky Bar-El M, Vankova P, Man P, Haitin Y*, Giladi M*. Structure of the human heterotetrameric cis-prenyltransferase complex. bioRxiv. 2020:095570. DOI: 10.1101/2020.06.02.095570.

  • Lisnyansky Bar-El M, Lee SY, Ki AY, Kapelushnik N, Loewenstein A, Chung KY, Schneidman-Duhovny D, Giladi M*, Newman H, Haitin Y*. Structural characterization of full-length human dehydrodolichyl diphosphate synthase using an integrative computational and experimental approach. bioRxiv. 2019:787119. DOI: 10.1101/787119.

  • Ben-Bassat A, Giladi M, Haitin Y. Structure of KCNH2 cyclic nucleotide-binding homology domain reveals a functionally vital salt-bridge. bioRxiv. 2019:790154. DOI: 10.1101/790154.

*,# Equal contribution 

 

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